Magnetic resonance imaging (MRI) is a popular imaging tool that is valuable for the early detection and monitoring of malignancies because it does not involve radiation and is noninvasive. Metal-based contrast agents (CAs) are commonly used in clinical settings despite concerns about the toxicity of free metals. Therefore, finding alternative nontoxic imaging probes is vital. In this work, we have synthesized and effectively utilized sustainable biomass lignin-based all-organic nanoconjugates linked with nitroxide radicals as MRI CAs. Lignin grafted with poly(4-glycidyloxy-2,2,6,6-tetramethylpiperidine-1-oxyl) (LPGT) exhibits a longitudinal relaxivity of 0.54 mM-1 s-1. LPGT shows exceptional characteristics, including resistance to reduction and nontoxicity toward living organisms. LPGT displays enhanced MRI contrast in the BALB/c mouse model for a duration exceeding 4.35 h. Our primary goal is to design MRI agents that are exceptionally effective sustainable biomass-derived materials and do not require the use of metals. Nicely, LPGT offers adequate contrast enhancement at 5-fold lower (0.020 mmol/kg) than the standard dose (0.1 mmol/kg), easing worries about toxic metal buildup. Consequently, LPGT shows promise as a feasible CA for metal-free MRI.
Keywords: biomass; contrast agents; lignin; magnetic resonance imaging; metal-free.