Dual-signal ratiometric electrochemical aptasensor for Zearalenone detection based on magnetic-assisted enrichment and hybridization chain reaction

Wentao Guo; Xuxin Zhang; Bin Deng; Hao Chen; Shun Wu; Yaohui Wu; Yonghong Wang; Ge Ning

doi:10.1016/j.foodchem.2024.141963

Dual-signal ratiometric electrochemical aptasensor for Zearalenone detection based on magnetic-assisted enrichment and hybridization chain reaction

Food Chem. 2024 Nov 8;465(Pt 1):141963. doi: 10.1016/j.foodchem.2024.141963. Online ahead of print.

Authors

Wentao Guo¹, Xuxin Zhang¹, Bin Deng¹, Hao Chen¹, Shun Wu¹, Yaohui Wu¹, Yonghong Wang², Ge Ning³

Affiliations

¹ Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation, Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha 410004, China.
² Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation, Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha 410004, China; Yuelushan Laboratory, Changsha 410004, China. Electronic address: bionano@163.com.
³ International Education Institute, Hunan University of Chinese Medicine, Changsha 410208, China.

PMID: 39531970
DOI: 10.1016/j.foodchem.2024.141963

Abstract

In this work, a dual-signal ratiometric electrochemical aptasensor based on the hybrid chain reaction (HCR) and streptavidin-modified magnetic beads (SA-MBs) was developed to rapidly detect zearalenone (ZEN). The HCR, as a powerful signal amplification technique to imporve the signal of sonser. When the target is present, they specifically bind with ZEN-Apt and release ZEN-cDNA to trigger HCR. Simultaneously, more double-stranded DNA causes the signal of Thi to be blocked. As a result, the two signals tend to change in the opposite direction as the ZEN concentration changes. Additionally, the peak current ratio of I_Thi/I_Fc showed a positive correlation with the ZEN concentration. Under optimal conditions, the constructed biosensor showed an excellent linear detection range (1.0 × 10^-10 mol/L to 1.0 × 10^-6 mol/L), a low detection limit (4.4 × 10^-11 mol/L) and high specificity for ZEN. In addition, the detection method retains the characteristics of low cost and rapid detection of electrochemical detection, while improving the detection limit and detection accuracy via SA-MBs and internal reference signal. This provides a new idea for the practical detection of ZEN.

Keywords: Aptamer; HCR; Magnetic beads; Ratiometric aptasensor; Zearalenone.