Tenebrio molitor (Coleoptera: Tenebrionidae) as an alternative host for the study of pathogenicity in Candida auris

Milena Izabel Dos Santos Rodrigues; Gabriel Henrique Rodrigues da Cruz; Fabíola Lucini; Alexandre Moreira de Almeida; Fabricio Fagundes Pereira; Rondon Tosta Ramalho; Simone Simionatto; Luana Rossato

doi:10.1016/j.micpath.2024.107115

Tenebrio molitor (Coleoptera: Tenebrionidae) as an alternative host for the study of pathogenicity in Candida auris

Microb Pathog. 2024 Nov 11:107115. doi: 10.1016/j.micpath.2024.107115. Online ahead of print.

Authors

Milena Izabel Dos Santos Rodrigues¹, Gabriel Henrique Rodrigues da Cruz¹, Fabíola Lucini¹, Alexandre Moreira de Almeida², Fabricio Fagundes Pereira², Rondon Tosta Ramalho³, Simone Simionatto¹, Luana Rossato⁴

Affiliations

¹ Health Science Research Laboratory, Universidade Federal da Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.
² Department of Biological and Environmental Science, Universidade Federal da Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.
³ Health and Development in the Midwest Region, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
⁴ Health Science Research Laboratory, Universidade Federal da Grande Dourados, Dourados, Mato Grosso do Sul, Brazil. Electronic address: luanarossato@ufgd.edu.br.

PMID: 39536838
DOI: 10.1016/j.micpath.2024.107115

Abstract

Background: Candida auris, a multidrug-resistant fungal pathogen, has emerged as a significant global health threat due to its high transmission and mortality rates, especially in healthcare settings.

Objective: This study aimed to establish the larvae of the coleopteran Tenebrio molitor (mealworm) as an in vivo model to evaluate the virulence of different C. auris strains.

Methods: T. molitor larvae were inoculated with varying doses and strains of C. auris. Mortality rates were monitored, melanization responses, and phenoloxidase activity were assessed. Histopathological analyses were conducted to observe tissue invasion by the yeast cells. Additionally, a biofilm formation test was included as a complementary analysis to determine if biofilm production would influence the virulence of the C. auris strains.

Results: A dose-dependent increase in mortality was observed, with the highest fungal load leading to the highest mortality rates. The study also revealed significant differences in virulence among the strains, with those from Kuwait and the reference strain CBS 10913 showing the highest pathogenicity. Melanization rates were significantly higher in infected larvae, indicating an active immune response. The histopathological analysis revealed the presence of C. auris cells within the tissue of T. molitor larvae. However, the biofilm formation complementary test did not show a significant difference in virulence among the different clades of C. auris.

Conclusion: The T. molitor model effectively demonstrated the pathogenic potential and virulence differences of C. auris strains. Strains from Kuwait and the reference strain CBS 10913 exhibited the highest virulence, causing 100% mortality within 24 hours. The model also highlighted significant biofilm formation and melanization responses, correlating with fungal burden. This insect model provides a valuable and cost-effective tool for preliminary virulence screening of clinical yeast strains, offering insights into host-pathogen interactions and the potential for evaluating antifungal treatments in vivo.

Keywords: Humoral response; insect model; mealworm; virulence.