Generation of the CTRL EiPS J9 mR6F-8 iPSC line derived from healthy human outgrowth blood endothelial cells (BOECs) using mRNA reprogramming methodology

Silvia Selvitella; Marta Pérez Franco; Begoña Aran; Anna Veiga; Bernd Kuebler

doi:10.1016/j.scr.2024.103606

Generation of the CTRL EiPS J9 mR6F-8 iPSC line derived from healthy human outgrowth blood endothelial cells (BOECs) using mRNA reprogramming methodology

Stem Cell Res. 2024 Nov 9:81:103606. doi: 10.1016/j.scr.2024.103606. Online ahead of print.

Authors

Silvia Selvitella¹, Marta Pérez Franco¹, Begoña Aran¹, Anna Veiga¹, Bernd Kuebler²

Affiliations

¹ Barcelona Stem Cell Bank, Spain; Regenerative Medicine Program, Institut d'Investigació Biomèdica, IDIBELL, Spain; P-CMR[C]-Program of Translation of Regenerative Medicine in Catalonia, Spain.
² Barcelona Stem Cell Bank, Spain; Regenerative Medicine Program, Institut d'Investigació Biomèdica, IDIBELL, Spain; P-CMR[C]-Program of Translation of Regenerative Medicine in Catalonia, Spain. Electronic address: bkuebler@idibell.cat.

PMID: 39541769
DOI: 10.1016/j.scr.2024.103606

Abstract

mRNA reprogramming is a technology for generating iPSCs with high efficiency and safety. However, it is not suitable for reprogramming non-adherent cells, the primary cell type in blood. An alternative is to obtain adherent cells from blood for this technology. To validate this approach, we generated a human iPSC line from blood outgrowth endothelial cells (BOECs) using mRNA-based reprogramming. The resulting line, CTRL EiPS J9 mR6F-8, meets iPSC criteria, including an undifferentiated state, pluripotency, genome integrity. This line is available upon request.