Construction of bilayer biomimetic periosteum based on SLA-3D printing for bone regeneration

Xingguo Zhou; Bin Zou; Qinghua Chen; Gongxian Yang; Qingguo Lai; Xinfeng Wang

doi:10.1016/j.colsurfb.2024.114368

Construction of bilayer biomimetic periosteum based on SLA-3D printing for bone regeneration

Colloids Surf B Biointerfaces. 2024 Nov 7:246:114368. doi: 10.1016/j.colsurfb.2024.114368. Online ahead of print.

Authors

Xingguo Zhou¹, Bin Zou², Qinghua Chen³, Gongxian Yang³, Qingguo Lai⁴, Xinfeng Wang³

Affiliations

¹ Center for Advanced Jet Engineering Technologies (CaJET), School of Mechanical Engineering, Shandong University, Jinan 250061, China; Key Laboratory of High Efficiency and Clean Mechanical Manufacture, Ministry of Education, Shandong University, China; National Demonstration Center for Experimental Mechanical Engineering Education (Shandong University), China; Additive Manufacturing Research Center of Shandong University of National Engineering Research Center of Rapid Manufacturing, Jinan 250061, China; Department of Gastrointestinal Surgery, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, China.
² Center for Advanced Jet Engineering Technologies (CaJET), School of Mechanical Engineering, Shandong University, Jinan 250061, China; Key Laboratory of High Efficiency and Clean Mechanical Manufacture, Ministry of Education, Shandong University, China; National Demonstration Center for Experimental Mechanical Engineering Education (Shandong University), China; Additive Manufacturing Research Center of Shandong University of National Engineering Research Center of Rapid Manufacturing, Jinan 250061, China. Electronic address: zb78@sdu.edu.cn.
³ Center for Advanced Jet Engineering Technologies (CaJET), School of Mechanical Engineering, Shandong University, Jinan 250061, China; Key Laboratory of High Efficiency and Clean Mechanical Manufacture, Ministry of Education, Shandong University, China; National Demonstration Center for Experimental Mechanical Engineering Education (Shandong University), China; Additive Manufacturing Research Center of Shandong University of National Engineering Research Center of Rapid Manufacturing, Jinan 250061, China.
⁴ Department of Oral and Maxillofacial Surgery, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, China; Research Center of 3D Printing in Stomatology of Shandong University, China.

PMID: 39547008
DOI: 10.1016/j.colsurfb.2024.114368

Abstract

An ideal biomimetic periosteum should have excellent biocompatibility to promote osteoclast adhesion and improve osseointegration, which is significant in promoting bone regeneration. In this work, a bionic artificial periosteum printed by the SLA-3D printing was prepared, consisting of a poly (ethylene glycol) diacrylate (PEGDA)/chitosan/tricalcium phosphate (TCP) fibrous layer and a gelatin methacryloyl (GelMA)/ammonium molybdate (Mo) cambium layer. Distinct surface characteristics were achieved on both sides of the biomimetic periosteum. Among them, the fibrous layer has high mechanical properties and low porosity, which is conducive to preventing the pulling of muscle tissues and the invasion of soft tissues. The cambium layer has a porous structure and bioactive factors that can effectively promote osteogenic differentiation of preosteoblasts. Combined with mild photothermal therapy triggered by NIR light, the biomimetic periosteum could promote bone regeneration at both the chemical and physical levels. This 3D-printed bilayer hydrogel can provide a promising strategy for preparing advanced tissue-engineered periosteum with excellent physical and bone regeneration properties.

Keywords: 3D printing; Biomimetic periosteum; Bone tissue engineering; Photothermal therapy.