Biophysical characterization of blood coagulation factor VIII binding to lipid nanodiscs that mimic activated platelet surfaces

Nathan G Avery; Isabelle R Young; Selena Lu; Jordan D Vaughan; Patrick S Korus; Tera N Richardson; Kenneth C Childers; Serge L Smirnov; P Clint Spiegel Jr

doi:10.1016/j.jtha.2024.11.003

Biophysical characterization of blood coagulation factor VIII binding to lipid nanodiscs that mimic activated platelet surfaces

J Thromb Haemost. 2024 Nov 14:S1538-7836(24)00690-1. doi: 10.1016/j.jtha.2024.11.003. Online ahead of print.

Authors

Nathan G Avery¹, Isabelle R Young¹, Selena Lu¹, Jordan D Vaughan¹, Patrick S Korus¹, Tera N Richardson¹, Kenneth C Childers¹, Serge L Smirnov¹, P Clint Spiegel Jr²

Affiliations

¹ Chemistry Department, Western Washington University, Bellingham, Washington, USA.
² Chemistry Department, Western Washington University, Bellingham, Washington, USA. Electronic address: Paul.Spiegel@wwu.edu.

PMID: 39549835
DOI: 10.1016/j.jtha.2024.11.003

Abstract

Background: Following proteolytic activation, activated blood coagulation factor VIII (FVIIIa) binds to activated platelet membranes, forming the intrinsic tenase complex with activated factor IX (FIXa). Previous studies have identified the C1 and C2 domains as the membrane binding domains of FVIII through conserved arginine residues. A membrane binding model for the FVIII C domains proposes that surface exposed hydrophobic and positively charged residues at each C domain interact with the membrane, yet a comprehensive thermodynamic and structural description of this interaction is lacking.

Objective: To determine residues of interaction, thermodynamics, and membrane binding preference for FVIII membrane association.

Methods: Binding of FVIII constructs to lipid nanodiscs were characterized with nuclear magnetic resonance (NMR), isothermal titration calorimetry (ITC), bio-layer interferometry (BLI), and X-ray crystallography.

Results: The thermodynamics of FVIII membrane binding indicate that the C1 domain associates through an enthalpically driven process while the C2 domain is entropically driven. Alanine mutations to surface-exposed hydrophobic residues in the C2 domain reveal differential effects on membrane binding, highlighting important determinants at the residue-level. The structure of a C2 double mutant, L2251A/L2252A, demonstrates that its decreased affinity is likely due to decreasing the surface area hydrophobicity. NMR studies with the C2 domain identified residues of interaction with soluble O-phospho-L-serine (OPLS) as well as lipid nanodiscs. Lastly, increasing phosphatidylethanolamine (PE) and decreasing PS content decreases overall FVIII affinity for membrane surfaces.

Conclusion: This study provides further insight into the molecular basis for how FVIII interacts with platelets to form the intrinsic tenase complex.

Keywords: blood coagulation; factor VIII; lipid membrane; protein/lipid interactions; thermodynamics.