Fasciolopsis buski isolated from human host, India has distinct clade based on nuclear ribosomal DNA sequences

Sandhya Chaurasiya; Aradhana Singh; Anurag Tiwari; Tuhina Banerjee

doi:10.1016/j.ijmmb.2024.100757

Fasciolopsis buski isolated from human host, India has distinct clade based on nuclear ribosomal DNA sequences

Indian J Med Microbiol. 2024 Nov 19:52:100757. doi: 10.1016/j.ijmmb.2024.100757. Online ahead of print.

Authors

Sandhya Chaurasiya¹, Aradhana Singh², Anurag Tiwari³, Tuhina Banerjee⁴

Affiliations

¹ Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India. Electronic address: nain.s9876@gmail.com.
² Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India. Electronic address: aradhanasingh.148@gmail.com.
³ Department of Gastroenterology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India. Electronic address: tiwarianuragk@gmail.com.
⁴ Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India. Electronic address: drtuhina105@bhu.ac.in.

PMID: 39550066
DOI: 10.1016/j.ijmmb.2024.100757

Abstract

Fasciolopsiasis caused by Fasciolopsis buski, is a significant cause of morbidity. In the present study F. buski detected during endoscopy of a 50-year-old male patient form Uttar Pradesh, India was used for phylogenetic analysis based on 28S rDNA and ITS2 regions. The parasite was genetically similar to an isolate of pig from Meghalaya. The intra-species genetic variation for 28S rDNA was 1-3% (India), 5% (Vietnam) and for ITS2, 1-2% (India) while 17 % from Vietnam. Thus, F. buski from humans or pigs from India were closely related than those from Vietnam and China which were grouped into separate clades.

Keywords: Clade; Distinct; Endemic; Fasciolopsiasis; Uttar Pradesh.