Differential scanning calorimetric domain dissection for HSA upon interaction with Bortezomib: Unveiling the binding dynamics

Bagher Davaeil; Anita Saremipour; Faezeh Moosavi-Movahedi; S Mohsen Asghari; Ali Akbar Moosavi-Movahedi

doi:10.1016/j.ijbiomac.2024.137728

Differential scanning calorimetric domain dissection for HSA upon interaction with Bortezomib: Unveiling the binding dynamics

Int J Biol Macromol. 2024 Nov 17;283(Pt 2):137728. doi: 10.1016/j.ijbiomac.2024.137728. Online ahead of print.

Authors

Bagher Davaeil¹, Anita Saremipour¹, Faezeh Moosavi-Movahedi¹, S Mohsen Asghari¹, Ali Akbar Moosavi-Movahedi²

Affiliations

¹ Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
² Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. Electronic address: moosavi@ut.ac.ir.

PMID: 39551302
DOI: 10.1016/j.ijbiomac.2024.137728

Abstract

Human serum albumin (HSA), a crucial plasma protein, plays a significant role in drug interactions within the bloodstream, bearing considerable clinical relevance. Bortezomib (BTZ) is a potent anti-cancer drug for multiple myeloma (MM) and mantle cell lymphoma (MC). The mechanism of BTZ transfer in the blood remains undetermined. This study aims to investigate the binding of BTZ to HSA using the techniques of differential scanning calorimetry (DSC), circular dichroism (CD), fluorescence spectroscopy, and computational methods such as molecular docking and molecular dynamics simulations. This study presents the thermal dissection of domain I (D_I) of HSA by subjecting it to a temperature elevation of 79.2 °C (2 °C above T_m of D_I) using DSC, which provides new information on the thermal behavior of HSA domains. Furthermore, the deconvolution analysis of the HSA thermogram in the absence and presence of BTZ revealed that the drug binding site is located in D_I and impacts D_II. The interaction between BTZ and HSA with a binding affinity (K_b) of 7.744±0.2 ×10⁵ M^-1 influences protein dynamics and reduces HSA's thermal stability by almost 1 °C. This study is crucial for predicting the pharmacokinetics and pharmacodynamics of BTZ, aiding in developing safer and more effective treatments for MM and MC.

Keywords: Bortezomib; Differential scanning calorimetry; HSA domain I dissection; HSA domains stability; HSA-Bortezomib interaction; Human serum albumin.