The Zymoseptoria tritici effector Zt-11 contributes to aggressiveness in wheat

PLoS One. 2024 Nov 19;19(11):e0313859. doi: 10.1371/journal.pone.0313859. eCollection 2024.

Abstract

Zymoseptoria tritici is an ascomycete fungus and the causal agent of Septoria tritici leaf blotch (STB) in wheat. Z. tritici secretes an array of effector proteins that are likely to facilitate host infection, colonisation and pycnidia production. In this study we demonstrate a role for Zt-11 as a Z. tritici effector during disease progression. Zt-11 is upregulated during the transition of the pathogen from the biotrophic to necrotrophic phase of wheat infection. Deletion of Zt-11 delayed disease development in wheat, reducing the number and size of pycnidia, as well as the number of macropycnidiospores produced by Z. tritici. This delayed disease development by the ΔZt-11 mutants was accompanied by a lower induction of PR genes in wheat, when compared to infection with wildtype Z. tritici. Overall, these data suggest that Zt-11 plays a role in Z. tritici aggressiveness and STB disease progression possibly via a salicylic acid associated pathway.

MeSH terms

  • Ascomycota* / genetics
  • Ascomycota* / pathogenicity
  • Ascomycota* / physiology
  • Fungal Proteins* / genetics
  • Fungal Proteins* / metabolism
  • Gene Expression Regulation, Fungal
  • Plant Diseases* / microbiology
  • Plant Leaves / metabolism
  • Plant Leaves / microbiology
  • Salicylic Acid / metabolism
  • Triticum* / genetics
  • Triticum* / microbiology

Substances

  • Fungal Proteins
  • Salicylic Acid

Supplementary concepts

  • Zymoseptoria tritici

Grants and funding

This research was funded by Science Foundation Ireland (SFI) career development award 15/CDA/3451, Strategic Partnerships Programme (16/SPP/3296) and Frontiers for the Future Programme 20/FFP-P/8545.