Ultrasonic-assisted plasma-activated water extraction of polysaccharide from Hemerocallis citrina Baroni: Structural characterization and antioxidant mechanism in vitro

Tongliang Yang; Han Zhang; Yaqing Bian; Zihan Qu; Yifu Zhang; Shuhong Li; Guiyun Chen; Ye Chen

doi:10.1016/j.foodchem.2024.142049

Ultrasonic-assisted plasma-activated water extraction of polysaccharide from Hemerocallis citrina Baroni: Structural characterization and antioxidant mechanism in vitro

Food Chem. 2024 Nov 14;465(Pt 2):142049. doi: 10.1016/j.foodchem.2024.142049. Online ahead of print.

Authors

Tongliang Yang¹, Han Zhang¹, Yaqing Bian¹, Zihan Qu¹, Yifu Zhang¹, Shuhong Li¹, Guiyun Chen¹, Ye Chen²

Affiliations

¹ State Key Laboratory of Food Nutrition and Safety; College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
² State Key Laboratory of Food Nutrition and Safety; College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: chenye@tust.edu.cn.

PMID: 39561598
DOI: 10.1016/j.foodchem.2024.142049

Abstract

Natural polysaccharides derived from Hemerocallis citrina Baroni exhibit significant biological activity. To enhance extraction efficiency and antioxidant activity, ultrasonic-assised plasma-activated water (PAW) was utilized to extract polysaccharides from Hemerocallis citrina Baroni. PAW demonstrated enhanced permeability and diffusion capabilities due to a reduced percentage of fully hydrogen-bonded structures, along with optimal pH (3.4) and conductivity (157.20 μS/cm). Compared to other methods, PAW significantly improved the extraction efficiency of polysaccharides to 38.24 %. Hemerocallis citrina Baroni polysaccharides predominantly consisted of α-pyranose sugars with sugar uronic acids, and ultrasound-assisted PAW did not alter their major functional groups. The extracted Hemerocallis citrina Baroni polysaccharides exhibited a semi-rigid triple-helical structure. Importantly, these polysaccharides displayed strong antioxidant activity, with ABTS⁺, DPPH, and O₂^- radical scavenging rates of 66.31 %, 65.98 %, and 47.73 % respectively. This study provides an effective method for extracting natural plant polysaccharides and offers valuable insights into their potential applications in the food industry and beyond.

Keywords: Antioxidant activity; Hemerocallis citrina Baroni polysaccharides; Structural characterization; Ultrasonic-assisted plasma-activated water.