A broad-spectrum antibody-based lateral flow immunoassay for detection of carbofuran and 3-hydroxy-carbofuran

Jinkui Wang; Tong Bu; Qianqian Cui; Zizhe Li; Qing Xu; Zhixian Jiang; Yexuan Mao; Xianqing Huang; Lianjun Song; Dapeng Peng; Xiya Zhang

doi:10.1016/j.foodchem.2024.142062

A broad-spectrum antibody-based lateral flow immunoassay for detection of carbofuran and 3-hydroxy-carbofuran

Food Chem. 2024 Nov 14;465(Pt 2):142062. doi: 10.1016/j.foodchem.2024.142062. Online ahead of print.

Authors

Affiliations

¹ Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control, College of Food Science and Technology, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan 450002, China.
² National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China.
³ Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control, College of Food Science and Technology, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan 450002, China. Electronic address: zhangxiya@henau.edu.cn.

PMID: 39561600
DOI: 10.1016/j.foodchem.2024.142062

Abstract

Simultaneous detection of carbofuran (CBF) and 3-hydroxy carbofuran (3-OH-CBF) in fruits and vegetables is important due to their high toxicity and widespread use in pest control. However, most lateral flow immunoassay (LFA) approaches only detection CBF. To overcome this limitation, two haptens, 6-((2,2-dimethyl-2,3-dihydrobenzofuran-7-yl)oxy)hexanoic acid and 6-((((2,2-dimethyl-2,3-dihydroben zofuran-7-yl)oxy)carbonyl)amino)hexanoic acid, named H1 and H2, were designed for broad-spectrum antibody detection of CBF and 3-OH-CBF. The highly specific monoclonal antibody (mAb) 3F4 based on hapten H1 only recognized CBF, while broad-spectrum mAbs 1D3, 6B7 and 6E8 based on hapten H2 recognized both compounds. The carbon bond to the hydroxyl group of 3-OH-CBF adopts a 28.55° angle relative to the same carbon bond of CBF, explaining the specificity of the H1-based mAb against CBF. After optimizing the antigen-antibody combination, a sensitive colloidal gold-LFA (CG-LFA) strip was constructed based on mAb 1D3 and H2-BSA for CBF and 3-OH-CBF detection following simple sample pretreatment. The established LFA had a limit of detection of 0.49-6.63 ng/mL, and adequate recoveries (69.3-105.7 %) for CBF and 3-OH-CBF in 26 fruit and vegetable samples.

Keywords: 3-hydroxy-carbofuran; Broad-spectrum antibody; CG-LFA; Carbofuran; Simultaneous detection.