Poly(3-hydroxybutyrate) (PHB) is crucial for replacing petroleum-based plastics, an essential step towards fostering a bio-based economy. This shift is urgently needed to safeguard human health and preserve natural ecosystems. PHB is one of the most extremely commercialized bio-plastics. Although. significant progress has been made in identifying bacteria that produce PHB, fewer bacteria capable of degrading it have been discovered. Four newly isolated Massilia strains capable of degrading PHB were discovered in eggplant (Solanum melongena L.) field soil. Their PHB-degrading abilities were investigated under different temperatures and media using emulsified solid-media based cultures. The strains belong to the genus Massilia, were evaluated for their effectiveness. Among them, Massilia sp. JJY02, was selected for its exceptional PHB degradation. PHB degradation was confirmed by monitoring changes in the physical and chemical properties of PHB films using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and gel permeation chromatography (GPC). After 20 days of cultivation with PHB film, Massilia sp. JJY02 achieved approximately 90% PHB degradation at 28 °C. All the novel strains were capable of producing carotenoid-type pigments and indole-acetic acid (IAA). Among them, Massilia sp. JJY03 and JJY04 showed phosphate solubilization activity. This study demonstrated that soil bacteria from eggplant have both PHB-degrading and plant growth promoting capabilities, marking the first instance of showing that species of Massilia can degrade PHB.
Keywords: Biodegradation; Carotenoid; Eggplant; Indole-acetic acid; Massilia sp.; Poly (3-hydroxybutyrate).
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