Diterpenoids and lignans from the stems of Tripterygium wilfordii and their anti-inflammatory activities

Yulu Tian; Yazi Wei; Haowen Luo; Xinyi Chen; Jie Ma; Yingda Zang; Tiantai Zhang; Dongming Zhang; Chuangjun Li

doi:10.1016/j.fitote.2024.106306

Diterpenoids and lignans from the stems of Tripterygium wilfordii and their anti-inflammatory activities

Fitoterapia. 2024 Nov 20:180:106306. doi: 10.1016/j.fitote.2024.106306. Online ahead of print.

Authors

Yulu Tian¹, Yazi Wei¹, Haowen Luo¹, Xinyi Chen¹, Jie Ma¹, Yingda Zang¹, Tiantai Zhang², Dongming Zhang³, Chuangjun Li⁴

Affiliations

¹ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
² State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: ttzhang@imm.ac.cn.
³ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: zhangdm@imm.ac.cn.
⁴ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: lichuangjun@imm.ac.cn.

PMID: 39571763
DOI: 10.1016/j.fitote.2024.106306

Abstract

Two undescribed diterpenoids (1-2) and three lignans (3a/3b, 4a), together with sixteen known compounds (4b, 5-9, 10a/10b-14a/14b) were obtained from the stems of Tripterygium wilfordii (T. wilfordii). The structures of these new compounds were elucidated by detailed spectroscopic analyses, and their absolute configurations were estabished by ECD calculations. Some compounds exhibited moderate inhibitory activities against IL-6 production in LPS-stimulated RAW 264.7 macrophages in vitro. Compound 5 showed significant anti-inflammatory activity in vitro (IC₅₀, 0.77 μM) and inhibited macrophage polarization towards a pro-inflammatory phenotype.

Keywords: Anti-inflammatory; Diterpenoids; Lignans; Stem; Tripterygium wilfordii.