First report of Peroxiredoxin-5 (PRDX5) in Starry Flounder (Platichthys stellatus): Molecular features and expression analysis

Min-Young Sohn; Kwang-Min Choi; Gyoungsik Kang; Won-Sik Woo; Kyung-Ho Kim; Ha-Jeong Son; Jun-Ichi Hikima; Chan-Il Park

doi:10.1016/j.dci.2024.105291

First report of Peroxiredoxin-5 (PRDX5) in Starry Flounder (Platichthys stellatus): Molecular features and expression analysis

Dev Comp Immunol. 2024 Nov 19:105291. doi: 10.1016/j.dci.2024.105291. Online ahead of print.

Authors

Min-Young Sohn¹, Kwang-Min Choi², Gyoungsik Kang¹, Won-Sik Woo¹, Kyung-Ho Kim¹, Ha-Jeong Son¹, Jun-Ichi Hikima³, Chan-Il Park⁴

Affiliations

¹ Department of Aquatic Life Medicine, College of Marine Science, Gyeongsang National University, 2 Tongyeonghaean-ro, Tongyeong 53064, Republic of Korea.
² Ecological Risk Research Department, Korea Institute of Ocean Science and Technology (KIOST), Geoje, 53201, Republic of Korea.
³ Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki, 889-2192, Japan. Electronic address: jhikima@cc.miyazaki-u.ac.jp.
⁴ Department of Aquatic Life Medicine, College of Marine Science, Gyeongsang National University, 2 Tongyeonghaean-ro, Tongyeong 53064, Republic of Korea. Electronic address: vinus96@hanmail.net.

PMID: 39571926
DOI: 10.1016/j.dci.2024.105291

Abstract

In this study, the Peroxiredoxin-5 (PRDX5) gene from starry flounder (Platichthys stellatus, PsPRDX5) was identified through next-generation sequencing (NGS) and verified via cloning and sequencing. The predicted amino acid sequence, derived from the cDNA, was confirmed by multiple sequence alignment and phylogenetic analysis, establishing its homology with related sequences. PsPRDX5 mRNA was expressed across all examined tissues in healthy starry flounder, with distinct tissue-specific expression profiles. Following artificial infection with viral hemorrhagic septicemia virus (VHSV) and Streptococcus parauberis PH0710, significant alterations in PsPRDX5 mRNA expression were observed. VHSV infection resulted in a pronounced downregulation of PsPRDX5 in most major tissues, except for a significant upregulation in the intestine at 7 days post-infection. Conversely, infection with S. parauberis PH0710 led to a significant upregulation of PsPRDX5 in nearly all tissues. These results suggest that the differential expression of PsPRDX5 in response to VHSV and S. parauberis PH0710 reflects pathogen-specific immune and antioxidant defense mechanisms, highlighting the role of PsPRDX5 in the immune response of starry flounder to both viral and bacterial infections.

Keywords: Immune response; PRDX5; Platichthys stellatus; Reactive oxygen species (ROS); Streptococcus parauberis; Viral haemorrhagic septicaemia virus.