Transcriptome and metabolome analyses provide crucial insights into the adaptation of chieh-qua to Fusarium oxysporum infection

Yanchun Qiao; Jiazhu Peng; Bei Wu; Min Wang; Guoping He; Qingwu Peng; Yin Gao; Yuping Liu; Songguang Yang; Xiuchun Dai

doi:10.3389/fpls.2024.1344155

Transcriptome and metabolome analyses provide crucial insights into the adaptation of chieh-qua to Fusarium oxysporum infection

Front Plant Sci. 2024 Nov 7:15:1344155. doi: 10.3389/fpls.2024.1344155. eCollection 2024.

Authors

Yanchun Qiao^#¹, Jiazhu Peng^#², Bei Wu^#¹, Min Wang³, Guoping He¹, Qingwu Peng³, Yin Gao⁴, Yuping Liu², Songguang Yang³, Xiuchun Dai¹

Affiliations

¹ Vegetable Science Department, Guangzhou Academy of Agricultural and Rural Sciences, Guangzhou, China.
² Vegetable Research Institute, Guangzhou Academy of Agricultural Sciences, Guangzhou, China.
³ Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
⁴ South China Agricultural University, College of Horticulture, Guangzhou, China.

^# Contributed equally.

Abstract

Introduction: Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) is a wax gourd variety that is generally susceptible to infection and damage by Fusarium oxysporum during its cultivation. Therefore, analyzing the adaption mechanism of chieh-qua to F. Oxysporum infection is of great significance for cultivating resistant varieties.

Methods: Through comparative transcriptome analysis, comparative metabolome analysis, integrated analysis of transcriptome and metabolome and between F. Oxysporum infected samples and control samples of susceptible lines.

Results: This study found that proteins such as NPR1, TGA and PR1 in plant hormone signal transduction pathway were up-regulated after infection, which may activate a series of plant secondary metabolic synthesis pathways. In addition, the expression of 27 genes in the flavonoid biosynthetic process in resistant lines after infection was significantly higher than that in susceptible lines, indicating that these genes may be involved in fungal resistance. This study also found that alternative splicing of genes may play an important role in responding to F. Oxysporum infection. For example, plant protein kinase genes such as EDR1, SRK2E and KIPK1 were not differentially expressed after F. Oxysporum infection, but the transcripts they produced differ at the transcription level. Finally, through comparative metabolome analysis, this study identified potentially functional substances such as oxalic acid that increased in content after F. Oxysporum infection. Through integrated analysis of transcriptome and metabolome, some differential expressed genes significantly related to differential metabolites were also identified.

Discussion: This study provides a basis for understanding and utilizing chieh-qua's infection mechanism of F. Oxysporum through analysis of the transcriptome and metabolome.

Keywords: Fusarium oxysporum; alternative splicing; chieh-qua; differentially expressed; metabolome.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the 2024 Special Seed Industry Revitalization Project of Rural Revitalization Strategy (2024-NPY-01-003), the Agricultural Support Fund Project of Guangzhou (23107180), the Science and Technology Program of Guangzhou (202002020086).