Enzymes are crucial for various technological applications, but their inherent instability and short lifespan pose challenges. This study presents facile immobilized enzyme technology with the development of thermoresponsive enzyme-polymer conjugates (EPCs), using glucose oxidase (GOx) as a model enzyme, to address these limitations. By conjugating heteropolymers to the glycan moieties of GOx through a precise in situ polymerization process, we could modulate the lower critical solution temperature of the EPCs, enhancing enzyme performance without compromising its active site. The EPCs demonstrate a switchable behavior that facilitates efficient homogeneous catalysis and easy heterogeneous separation, reducing costs and environmental impact in industrial applications. Our strategy presents a versatile platform for creating efficient biocatalysts with tunable properties, marking a step forward in sustainable and cost-effective bioprocessing.