Biosensors with vancomycin and polymetallic metal-organic frameworks for colorimetric-fluorescent dual-mode detection and sterilization of bacteria

Wei Chen; Xiayu Peng; Lichao Kang; Shengnan Dong; Jian Zhang; Yunfeng Zhao; Fengxia Sun

doi:10.1016/j.jhazmat.2024.136582

Biosensors with vancomycin and polymetallic metal-organic frameworks for colorimetric-fluorescent dual-mode detection and sterilization of bacteria

J Hazard Mater. 2024 Nov 19:482:136582. doi: 10.1016/j.jhazmat.2024.136582. Online ahead of print.

Authors

Wei Chen¹, Xiayu Peng², Lichao Kang³, Shengnan Dong⁴, Jian Zhang⁴, Yunfeng Zhao¹, Fengxia Sun⁵

Affiliations

¹ School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China; Key Laboratory of Agricultural Product Processing and Quality Control of Specialty(Co-construction by Ministry and Province), School of Food Science and Technology, Shihezi University, Shihezi, China.
² College of Animal Science and Technology, Shihezi University, Shihezi, China. Electronic address: pengxy@shzu.edu.cn.
³ Analysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, China.
⁴ School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China; Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi, China.
⁵ School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China; Key Laboratory of Agricultural Product Processing and Quality Control of Specialty(Co-construction by Ministry and Province), School of Food Science and Technology, Shihezi University, Shihezi, China; Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi, China. Electronic address: shzsfx@163.com.

PMID: 39577286
DOI: 10.1016/j.jhazmat.2024.136582

Abstract

The development of a versatile platform for bacterial assay and elimination is urgently needed due to the danger that bacteria pose to human life. Here, we synthesized a trimetallic deposition and horseradish peroxidase (HRP)-embedded porous coordination network-224 hybrid nanozymes (PCN-224 @AuPdPt@HRP) with outstanding peroxidase activity and fluorescence quenching ability. On this basis, we designed a dual recognition strategy-driven colorimetric-fluorescence dual-mode detection platform using Listeria monocytogenes as a pattern analyte. The platform consisted of an aptamer-modified PCN-224 @AuPdPt@HRP (PCN-224 @AuPdPt@HRP@Aptamer) specifically recognizing Listeria monocytogenes and vancomycin-coated 96-well plates. In the presence of vancomycin, which has the ability to recognize and inactivate gram-positive bacteria, the significant peroxidase activity of PCN-224 @AuPdPt@HRP@Aptamer in the precipitate was able to catalyze the color change of the substrate by H₂O₂. Meanwhile, the residual PCN-224 @AuPdPt@HRP@Aptamer in the supernatant was able to change the fluorescence of fluorescein-labeled deoxyribonucleic acid (FAM-DNA). In summary, this paper presents a multifunctional platform capable of detecting and eliminating residual bacteria in real environments. This strategy is expected to facilitate the development of multifunctional biosensors based on metal-organic framework probes and also provide environmental health.

Keywords: Bacteria; Colorimetric; Dual-mode detection; Fluorescence; Sterilization.