Ferulic acid mitigated rotenone toxicity -Evoked Parkinson in rat model by featuring apoptosis, oxidative stress, and neuroinflammation signaling

Ola Mohammed Youssef; Nermeen Hosney Lashine; Mohammad El-Nablaway; Mona Ibrahim El-Yamany; Manar Monir Youssef; Dina Abdalla Arida

doi:10.1016/j.tice.2024.102614

Ferulic acid mitigated rotenone toxicity -Evoked Parkinson in rat model by featuring apoptosis, oxidative stress, and neuroinflammation signaling

Tissue Cell. 2024 Nov 16:91:102614. doi: 10.1016/j.tice.2024.102614. Online ahead of print.

Authors

Ola Mohammed Youssef¹, Nermeen Hosney Lashine¹, Mohammad El-Nablaway², Mona Ibrahim El-Yamany³, Manar Monir Youssef⁴, Dina Abdalla Arida⁵

Affiliations

¹ Human Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.
² Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Diriyah, Riyadh 13713, Saudi Arabia; Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: medo_bio@mans.edu.eg.
³ Department of Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Damietta University, New Damietta City, Egypt. Electronic address: monaelyamany@du.edu.eg.
⁴ Department of Molecular Biology, Faculty of Medicine, Cairo University, Egypt. Electronic address: Manarmoni1989@cu.edu.eg.
⁵ Human Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: drdinataher@mans.edu.eg.

PMID: 39577325
DOI: 10.1016/j.tice.2024.102614

Abstract

Over time, Parkinson disease (PD) develops as a neurological illness. The goal of this study was to see whether ferulic acid has any neuroprotective benefits on the cerebellum of rats that have Parkinson's disease brought on by rotenone poisoning. A total of twenty-four male albino rats, in good condition, weighed between 200 and 250 g and nine to ten weeks old, were employed in the investigation. The control group received 1 ml of sunflower oil intraperitoneally (i.p.) each day. Rats' motor performance was considerably worse when given rotenone than it was in the control group. Rats given Ferulic Acid (FA) showed a substantial drop in the amount of glutathione (GSH) in the cerebellum. Moreover, the injection of FA resulted in a significant reduction in the optical density (OD) of the immune-positive reaction for α-synuclein, and the area percentage of BCL-2 and NF-kB immunological positive response. FA therapy, surprisingly, enhanced the OD of TH immunopositive response and apoptotic regulators (BCL2) in the cerebellum. Furthermore, FA boosted BCL2 expression, confirming the antiapoptotic effects of FA. Based on these results, FA is probably a good candidate to treat neurodegenerative diseases brought on by long-term exposure to rotenone.

Keywords: Apoptosis; Ferulic acid; Oxidative stress; Parkinson; Rotenone.