H-NS-Mediated Regulation of Swimming Motility and Polar Flagellar Gene Expression in Vibrio parahaemolyticus

Abstract

Vibrio parahaemolyticus is equipped with two distinct flagellar systems: a polar flagellum and numerous lateral flagella. The polar flagellum plays a role in propelling swimming in liquids, while the lateral flagella serve to enhance swarming on surfaces or in viscous environments. H-NS is a histone-like nucleoid structuring protein that plays a regulatory role in both the swimming and swarming motility of V. parahaemolyticus. However, the detailed mechanisms have not been fully understood. In this study, we have demonstrated that the deletion of hns hindered the growth rate of V. parahaemolyticus during the logarithmic growth phase and significantly decreased the swimming motility. H-NS directly activated the transcription of flgMN, flgAMN, flgBCDEFGHIJ, and flgKL-flaC located within the polar flagellar gene clusters. The expression of H-NS in Escherichia coli led to a marked elevation in the expression levels of flgM, flgA, flgB, and flgK, suggesting the positive effect of H-NS on the expression of polar flagellar genes in E. coli. This work demonstrates that the positive regulation of H-NS on the swimming motility in V. parahaemolyticus may be achieved through its regulation of polar flagellar gene expression and bacterial growth.