Immunophenotyping of Leukocytes in Brain in Hypothyroid Mice

Abstract

Hypothyroidism, characterized by inadequate production of thyroid hormones, and malaria, a mosquito-borne infectious disease caused by Plasmodium parasites, are significant health concerns worldwide. Understanding the interplay between these two conditions could offer insights into their complex relationship and potential therapeutic strategies. To induce hypothyroidism, pharmacological inhibition of thyroid hormone synthesis was employed. Subsequently, mice were infected with Plasmodium berghei ANKA to simulate cerebral malaria infection. It needs to monitor the progression of the disease in male mice before it can identify infiltrating immune system populations of interest in the brain by multiparametric techniques such as flow cytometry.

Keywords: Cerebral malaria; Flow cytometry; Hypothyroidism; Leukocytes; Plasmodium berghei ANKA.