Paraprobiotic Levilactobacillus brevis KU15151 exhibits antioxidative and anti-inflammatory activities in LPS-induced A549 cells

Cho Eun Kang; Ji Hun Kim; Na-Kyoung Lee; Hyun-Dong Paik

doi:10.1016/j.micpath.2024.107143

Paraprobiotic Levilactobacillus brevis KU15151 exhibits antioxidative and anti-inflammatory activities in LPS-induced A549 cells

Microb Pathog. 2024 Nov 21:198:107143. doi: 10.1016/j.micpath.2024.107143. Online ahead of print.

Authors

Cho Eun Kang¹, Ji Hun Kim¹, Na-Kyoung Lee¹, Hyun-Dong Paik²

Affiliations

¹ Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, Republic of Korea.
² Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, Republic of Korea. Electronic address: hdpaik@konkuk.ac.kr.

PMID: 39579943
DOI: 10.1016/j.micpath.2024.107143

Abstract

Levilactobacillus brevis KU15151, isolated from kimchi, has been reported in previous studies to possess probiotic properties. Here, we sought to explore the potential of heat-killed L. brevis KU15151 in improving respiratory health by identifying its antioxidant and anti-inflammatory effects in LPS-induced A549 cells. Inactivated L. brevis KU15151 exhibited strong DPPH and ABTS radical-scavenging abilities (48.78 ± 3.95 % and 69.08 ± 1.09 %) and effectively reduced the production of reactive oxygen species (25.32 %). In addition, it was found to have anti-inflammatory effects by inhibiting phosphorylation of ERK 1/2 (0.556), JNK (0.476), p38 MAPK (0.580), p65 (0.579), and IκB-α (1.170), which are involved in MAPK and NF-κB signaling. It also suppressed the mRNA expression of pro-inflammatory cytokines (0.173-0.617), which are important factors in respiratory diseases. IL-6 (19.47 %) and eotaxin (50.19 %) levels were reduced as measured by ELISA. Therefore, heat-killed L. brevis KU15151 is expected to improve respiratory health.

Keywords: Anti-inflammation; Antioxidant; MAPK/NF-κB signaling; Paraprobiotics; Respiratory health.