Characterization of a Novel Tn7-like Transposon Carrying blaDHA-1 in Providencia stuartii MF1 Isolated from Swine Wastewater

Curr Microbiol. 2024 Nov 23;82(1):6. doi: 10.1007/s00284-024-03952-x.

Abstract

Providencia stuartii is an emerging pathogen that causes nosocomial infections. In this study, a multidrug-resistant strain P. stuartii MF1 was isolated from swine wastewater. Comprehensive analysis of whole genome sequencing revealed that dozens of antibiotic resistance genes were found in MF1. A novel transposon Tn6450M which has high sequence identity to Tn6450 and the plasmid-borne Tn6765 from Proteus mirabilis was identified in the genome of MF1. Tn6450M was determined to be stably inserted into a new attTn7 site in the P. stuartii MF1 genome and contains the third-generation cephalosporins resistance-associated genes blaDHA-1. Intergeneric transmission of Tn6450 variants poses risks for the spread of antibiotic resistance genes.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • DNA Transposable Elements*
  • Drug Resistance, Multiple, Bacterial* / genetics
  • Genome, Bacterial
  • Microbial Sensitivity Tests
  • Plasmids / genetics
  • Providencia* / drug effects
  • Providencia* / genetics
  • Providencia* / isolation & purification
  • Swine
  • Wastewater* / microbiology
  • Whole Genome Sequencing
  • beta-Lactamases / genetics

Substances

  • DNA Transposable Elements
  • Wastewater
  • Anti-Bacterial Agents
  • beta-Lactamases
  • Bacterial Proteins

Supplementary concepts

  • Providencia stuartii