A comparative investigation on the extraction-function relationship of polysaccharides derived from moringa oleifera seeds in terms of antioxidant capacity

Hongru Yang; Chongbin Lei; Dongyao Li; Na Zhang; Yumiao Lang; Liping Wu; Miaoshu Wang; Hongtao Tian; Chen Li

doi:10.1016/j.foodchem.2024.142137

A comparative investigation on the extraction-function relationship of polysaccharides derived from moringa oleifera seeds in terms of antioxidant capacity

Food Chem. 2024 Nov 20;465(Pt 2):142137. doi: 10.1016/j.foodchem.2024.142137. Online ahead of print.

Authors

Hongru Yang¹, Chongbin Lei², Dongyao Li², Na Zhang³, Yumiao Lang⁴, Liping Wu⁵, Miaoshu Wang⁶, Hongtao Tian⁷, Chen Li⁸

Affiliations

¹ College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei, 071000, China; College of Public Health, Hebei University, Baoding, Hebei, 071000, China.
² College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei, 071000, China.
³ College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei, 071000, China; College of Biochemistry and Environmental Engineering, Baoding University, Baoding, Hebei, 071000, China.
⁴ College of Public Health, Hebei University, Baoding, Hebei, 071000, China.
⁵ College of Nursing, Hebei University, Baoding, Hebei, 071000, China.
⁶ New Hope Tensun (Hebei) Dairy Co. Ltd., Baoding, Hebei, 071000, China; Hebei Technology Innovation Center of Probiotic Functional Dairy Product, Baoding, Hebei, 071000, China.
⁷ College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei, 071000, China; National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, Hebei, 071000, China; Hebei Technology Innovation Center of Probiotic Functional Dairy Product, Baoding, Hebei, 071000, China. Electronic address: tht631022@163.com.
⁸ College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei, 071000, China; Hebei Technology Innovation Center of Probiotic Functional Dairy Product, Baoding, Hebei, 071000, China. Electronic address: yitai123@163.com.

PMID: 39581097
DOI: 10.1016/j.foodchem.2024.142137

Abstract

Owing to the hypoglycemic, anti-inflammatory, and other beneficial bioactivities of Moringa oleifera seeds (MOS), the antioxidant activities and physicochemical characteristics of deproteinized polysaccharides extracted using hot water (MSWP) and alkali (MSAP) from MOS were systematically studied. MSAP exhibited higher antioxidant activity than MSWP in vitro. Subsequently, the antioxidant capacity was evaluated after oral administration of MSAP in diabetic rats. The results demonstrated a statistically significant increase in serum antioxidant parameters. The two polysaccharides were then purified and characterized. The molecular weights of purified fractions MSAP3 and MSWP1 were 4.582 × 10⁴ Da and 1.9058 × 10⁵ Da, respectively; the content of uronic acid in MSAP3 was higher than that in MSWP1; and both compounds presented typical infrared spectral absorption peaks of polysaccharides. In conclusion, MSAP exhibits superior antioxidant properties that depend on its structural characteristics, and this study provides data support for further elucidation of the hypoglycemic mechanism of MSAP.

Keywords: Antioxidant activity; Diabetic rat; Monosaccharide composition; Moringa oleifera seed; Polysaccharide.