A newly identified photosystem II Subunit P gene TaPsbP4A-1 in Triticeae species negatively regulates wheat powdery mildew resistance

Jun Xu; Mengfei Wang; Yueming Ren; Wanglong Luo; Lu Zhang; Shuangwei Liu; Ping Hu

doi:10.3389/fpls.2024.1452281

A newly identified photosystem II Subunit P gene TaPsbP4A-1 in Triticeae species negatively regulates wheat powdery mildew resistance

Front Plant Sci. 2024 Nov 8:15:1452281. doi: 10.3389/fpls.2024.1452281. eCollection 2024.

Authors

Jun Xu¹, Mengfei Wang², Yueming Ren², Wanglong Luo², Lu Zhang², Shuangwei Liu¹, Ping Hu²

Affiliations

¹ College of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, China.
² College of Agriculture, Henan Engineering Research Center of Crop Genome Editing/Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, Henan Institute of Science and Technology, Xinxiang, China.

Abstract

The photosystem II (PSII) Subunit P (PsbP) protein is a component of its oxygen-evolving complex, which can oxidize water to produce oxygen using light energy and is critical to the core components and stability of PSII. Using the whole-genome information, the PsbP genes of 10 plant species were comprehensively identified. The expression patterns of wheat PsbPs under Blumeria graminis f. sp. tritici (Bgt) infection were assessed using qRT-PCR, and the functions of TaPsbPs in wheat powdery mildew resistance were studied using barley stripe mosaic virus-induced gene silencing. In total, 122 PsbP genes were divided into 8 classes with similar gene structures. No tandem repeat events were identified in wheat PsbP, suggesting that the PsbP genes in common wheat were donated by its diploid progenitor species. The expression levels of TaPsbP2A-1, TaPsbP3A-1, TaPsbP4A-1, TaPsbP4A-2, and TaPsbP7A-2 were induced by Bgt. The silencing of TaPsbP4A-1 increased the resistance of common wheat 'Bainong AK58' to Bgt. This study provides valuable information for functional and evolutionary research on the PsbP gene family.

Keywords: barley stripe mosaic virus-induced gene silencing; evolutionary progress; expression pattern; photosystem II Subunit P; wheat powdery mildew.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was funded by the Basic Research Special Project of Key Research Projects in Higher Education Institutions of Henan Province (No. 24ZX013), the National Natural Science Foundation of China (No. 31901538).