Quantification of Neutralizing Antibodies in Serum Using VSV-MARV-GFP

Methods Mol Biol. 2025:2877:355-360. doi: 10.1007/978-1-0716-4256-6_24.

Abstract

Neutralizing antibodies play an important role in protection against filoviruses. However, the technique to assess neutralization titers with a replicating BSL-4 virus is limited. This chapter explains how to determine the neutralization titer in serum samples against Marburg virus (MARV). This method uses a replicating recombinant vesicular stomatitis virus expressing the MARV glycoprotein and the green fluorescent protein as a surrogate approach.

Keywords: Filovirus; Glycoprotein; Marburg virus; Neutralization; Vesicular stomatitis virus.

MeSH terms

  • Animals
  • Antibodies, Neutralizing* / blood
  • Antibodies, Neutralizing* / immunology
  • Antibodies, Viral* / blood
  • Antibodies, Viral* / immunology
  • Green Fluorescent Proteins* / genetics
  • Green Fluorescent Proteins* / metabolism
  • Humans
  • Marburgvirus* / genetics
  • Marburgvirus* / immunology
  • Neutralization Tests* / methods
  • Vesicular stomatitis Indiana virus / genetics
  • Vesicular stomatitis Indiana virus / immunology
  • Vesiculovirus / genetics
  • Vesiculovirus / immunology

Substances

  • Antibodies, Neutralizing
  • Green Fluorescent Proteins
  • Antibodies, Viral