Electrospinning of Poly-3-Hydroxybutyrate Fibers Loaded with Chlorophyll for Antibacterial Purposes

Polina M Tyubaeva; Ivetta A Varyan; Roman R Romanov; Vasily A Merzlikin; Olga A Gruznova; Dmitry V Gruznov; Nikolay I Popov; Gulizar Sh Shcherbakova; Ekaterina N Shuteeva; Irina P Chesnokova; Anton V Lobanov; Anatoly A Olkhov

doi:10.3390/polym16223221

Electrospinning of Poly-3-Hydroxybutyrate Fibers Loaded with Chlorophyll for Antibacterial Purposes

Polymers (Basel). 2024 Nov 20;16(22):3221. doi: 10.3390/polym16223221.

Authors

Polina M Tyubaeva^{1

2}, Ivetta A Varyan^{1

2}, Roman R Romanov², Vasily A Merzlikin¹, Olga A Gruznova^{3

4}, Dmitry V Gruznov⁵, Nikolay I Popov⁴, Gulizar Sh Shcherbakova⁴, Ekaterina N Shuteeva⁴, Irina P Chesnokova^{3

6}, Anton V Lobanov⁷, Anatoly A Olkhov^{1

2}

Affiliations

¹ Department of Physical Chemistry of Synthetic and Natural Polymer Compositions, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina St., 119334 Moscow, Russia.
² Academic Department of Innovational Materials and Technologies Chemistry, Plekhanov Russian University of Economics, 36 Stremyanny Per., 117997 Moscow, Russia.
³ Laboratory of Liquid-Phase Oxidation, Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 4 Kosygina Street, 119334 Moscow, Russia.
⁴ Laboratory of Veterinary Sanitation, All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology-Branch of Federal State Budget Scientific Institution "Federal Scientific Center-K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences", 5 Zvenigorodskoye Highway, 123022 Moscow, Russia.
⁵ Laboratory of Veterinary Sanitation and Environmental Safety in Beekeeping, All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology-Branch of Federal State Budget Scientific Institution "Federal Scientific Center-K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences", 5 Zvenigorodskoye Highway, 123022 Moscow, Russia.
⁶ Department of Chemistry and Technology of High-Molecular Compounds Named After S.S. Medvedev, MIREA-Russian Technological University, 78 Vernadsky Avenue, 119454 Moscow, Russia.
⁷ Department of General Chemistry, Moscow Pedagogical State University, 1/1 Malaya Pirogovskaya Street, 119435 Moscow, Russia.

Abstract

This work is devoted to the creation of biocompatible fibrous materials with a high antimicrobial effect based on poly-3-hydroxybutyrate (PHB) and chlorophyll (Chl). The data obtained show the possibility of obtaining fibrous materials from PHB and Chl by electrospinning methods. The obtained electrospun matrices were investigated by the SEM, DSC and FTIR methods. Various key properties of the matrices were evaluated, including hydrophilicity and mechanical strength, as well as photodynamic and light-dependent antimicrobial effects against the conditionally pathogenic microorganism Staphylococcus aureus. The results demonstrate a significant improvement in electrospinning properties for a concentration of 0.5% Chl and a reduction in fiber formation defects, as well as an increase in the strength of nonwovens. It was found that the antimicrobial potential of Chl-PHB (with concentrations of Chl of 1.25 and 1.5%) is higher than that of Chl in free form. It was also determined that irradiation increases the inhibitory effect of Chl, both in free form and in the form of a complex with a polymer.

Keywords: antibacterial properties; chlorophyll; electrospinning; poly-3-hydroxybutyrate.

Grants and funding

The work was carried out within the framework of the project “Research on the problems of recycling waste of natural origin for the practical use of the products obtained”(122122600056-9).