The incorporation a "singlet oxygen (1O2) battery" into photodynamic therapy (PDT) could overcome the deficiency of tumor hypoxia in PDT and enhance its effect. However, real-time monitoring the 1O2 release efficiency of the 1O2 battery still presents a significant challenge in vivo. To address this issue, we have developed a bright aggregation-induced emission (AIE) chemiluminescence (CL) probe (DTLum), which conjugates a luminol unit with a donor-acceptor structured diketopyrrolopyrrole fluorophore, for the specific detection of 1O2. Subsequently, the DTLum nanoparticles (DTLum NPs) were prepared using PEO100-PPO65-PEO100 (Pluronic F127) as the surfactant. The DTLum NPs can detect 1O2 in aqueous solution with a bright near-infrared (NIR) CL signal (651 nm) and great tissue penetration (12.5 mm), making them suitable for the detection of 1O2 both in vitro (quantitative) and in vivo (qualitative). Notably, by utilizing the DTLum NPs, the process of 1O2 release in 1O2 batteries with different release rates can be visually monitored in cells and in vivo. This NIR CL probe provides a powerful platform for real-time monitoring and evaluating the release efficiency of 1O2 battery.
Keywords: Aggregation-induced emission; Chemiluminescence imaging; Deep-tissue; Near-infrared; Singlet oxygen battery.
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