Biostimulants for enhancing productivity, bioactive components, and the essential oils of garlic with the potential antifungal activity

Hanaa S Hassan; Mostafa N Feleafel; Mina S R Abd El-Lahot; Mervat El-Hefny; Taghreed F M Abdel Rahman; Abeer A Mohamed; Doaa Y Abd-Elkader; R M Mahdy

doi:10.1186/s13568-024-01790-5

Biostimulants for enhancing productivity, bioactive components, and the essential oils of garlic with the potential antifungal activity

AMB Express. 2024 Nov 27;14(1):130. doi: 10.1186/s13568-024-01790-5.

Authors

Hanaa S Hassan¹, Mostafa N Feleafel¹, Mina S R Abd El-Lahot², Mervat El-Hefny³, Taghreed F M Abdel Rahman⁴, Abeer A Mohamed⁵, Doaa Y Abd-Elkader¹, R M Mahdy⁶

Affiliations

¹ Department of Vegetable, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, 21545, Egypt.
² Department of Food Science and Technology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, 21545, Egypt.
³ Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, 21545, Egypt. mervat.mohamed@alexu.edu.eg.
⁴ Department of Ornamental, Medicinal and Aromatic Plant Diseases, Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, 12619, Egypt.
⁵ Plant Pathology Institute, Agricultural Research Center (ARC), Alexandria, 21616, Egypt.
⁶ Horticulture Department, Faculty of Agriculture, Tanta University, Tanta, 31527, Egypt.

Abstract

To feed the world's growing population, the agriculture sector has recently had to strike a balance between reducing its detrimental effects on ecosystems and human health and boosting resource efficiency and production. In reality, pesticides and fertilizers are vital to agriculture and are useful instruments that farmers can employ to increase yield and guarantee steady productivity throughout the seasons under both favorable and unfavorable conditions. Therefore, in the present study, fertilizing with potassium citrate as a foliar spray and humic acid (HA) as a soil application allowed for the evaluation of vegetative growth parameters (plant height, number of leaves/plant), total phenolic content, total carbohydrate, antioxidant activity, the essential oil (EO) composition, and bulb yield of garlic (Allium sativum L.). These were carried out in two field experiments throughout the 2020-2021 and 2021-2022 growth seasons. A gas chromatography-mass spectroscopy (GC-MS) apparatus was performed to determine the chemical composition of the isolated EOs. The antifungal activity of the EOs was assessed against two fungi, Fusarium proliferatum and Macrophomina phaseolina, that cause geranium plants to wilt and decay. The findings indicated that applying HA at a rate of 2 g/L with potassium citrate at a rate of 5 or 10 mL/L produced garlic bulbs with the highest levels of productivity and diameter. The diverse treatments between HA with potassium citrate resulted in significant variations in the bioactive components, such as total phenol content, antioxidant activity, total carbohydrate, and sulfur content. The analysis of the EOs revealed the presence of dimethyl trisulfide, diallyl disulfide, methyl 2-propenyl trisulfide, allitridin, and methyl allyl disulfide and allyl tetrasulfide as main compounds. By gradually increasing the concentration of the garlic EO to 4000 µg/mL compared to the control, the inhibition percentage of fungal growth of F. proliferatum and M. phaseolina was increased. In conclusion, a high concentration of HA with potassium citrate (5 or 10 mL/L), may be suitable and highly appreciated as a fertilizer application to enhance the productivity and EOs content of garlic plants.

Keywords: Essential oils; Foliar potassium fertilizer; Fungal inhibition; Garlic; Humic acid; Productivity.