Janus radial nanofiber patch with leak-proof, antimicrobial, and osteogenic integration for skull base reconstruction

Xiaomei Xia; Zhen Yang; Hongshui Wang; Werner E G Müller; Xiaohong Wang; Lei Yang; Huan Zhou; Yi Xia; Chunyong Liang

doi:10.1016/j.bioadv.2024.214122

Janus radial nanofiber patch with leak-proof, antimicrobial, and osteogenic integration for skull base reconstruction

Biomater Adv. 2024 Nov 19:168:214122. doi: 10.1016/j.bioadv.2024.214122. Online ahead of print.

Authors

Xiaomei Xia¹, Zhen Yang², Hongshui Wang³, Werner E G Müller⁴, Xiaohong Wang⁴, Lei Yang², Huan Zhou⁵, Yi Xia⁶, Chunyong Liang⁷

Affiliations

¹ School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, China.
² School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, China; Center for Health Science and Engineering, Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300131, China.
³ Center for Health Science and Engineering, Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300131, China.
⁴ ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, D-55128 Mainz, Germany.
⁵ School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, China; Center for Health Science and Engineering, Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300131, China. Electronic address: zhouhuan@hebut.edu.cn.
⁶ Center for Health Science and Engineering, Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300131, China. Electronic address: 2022018@hebut.edu.cn.
⁷ School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, China. Electronic address: liangchunyong@hebut.edu.cn.

PMID: 39608190
DOI: 10.1016/j.bioadv.2024.214122

Abstract

During transsphenoidal surgery to remove pituitary adenomas, the structures of the skull base consisting of the dura mater and skull base bones are destroyed, making it crucial to restore the natural structure of the skull base. We crafted a dual-layer Janus fiber membrane utilizing the layer-by-layer electrospinning technique, comprising an osteoblast layer and a leak-proof antimicrobial layer. Specifically, RPG-1%PCPP radially aligned nanofibrous membranes (osteoblasts) can promote directional cell migration and facilitate cellular osteogenic differentiation. TPC nanofibrous membranes (leak-proof antimicrobial layer) can prevent fluid leakage while releasing antimicrobials for resisting bacterial infections. Bacteriostatic tests showed that cefazolin had a good inhibitory ability against both E. coli and S. aureus. RT-PCR showed that radial fiber membrane loaded with PCPP promoted the expression of ALP and BMP-2 genes, thereby promoting osteogenic differentiation of cells. Animal experiments showed that the BV/TV in the TPC/RPG-1%PCPP fiber membrane group was significantly higher than that in the blank group, indicating that TPC/RPG-1%PCPP could promote bone tissue regeneration.

Keywords: Antimicrobial testing; Cell migration; Osteogenic differentiation; Skull base reconstruction.