NRAV promoted the malignant progression of gastric cancer

Yuchen Wu; Dongdong Wang; Jie Zhao; Jinhui Guo; Zhenyu Gao; Qiuran Xu; Xiaoge Hu

doi:10.1016/j.gene.2024.149134

NRAV promoted the malignant progression of gastric cancer

Gene. 2024 Nov 28:149134. doi: 10.1016/j.gene.2024.149134. Online ahead of print.

Authors

Yuchen Wu¹, Dongdong Wang², Jie Zhao³, Jinhui Guo⁴, Zhenyu Gao⁵, Qiuran Xu⁶, Xiaoge Hu⁷

Affiliations

¹ The Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, China; Bengbu Medical University, Bengbu 233030, China.
² Jinzhou Medical University, Jinzhou 121001, China.
³ College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
⁴ Qingdao Medical College, Qingdao University, Qingdao 266071, China.
⁵ Wenzhou Medical University, Wenzhou 325035, China.
⁶ The Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, China. Electronic address: xuqiuran@hmc.edu.cn.
⁷ The Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, China. Electronic address: huxiaoge2008xmu@163.com.

PMID: 39615808
DOI: 10.1016/j.gene.2024.149134

Abstract

Gastric cancer (GC) has been ranked as the third incidence tumors globally. Long non-coding RNA (lncRNA) NRAV has been reported as a tumor-enhancer in the development of human cancers, whereas the function of NRAV in GC remains to be elucidated. The aim of this research was to explore the underlying function of NRAV in GC. Through comprehensive bioinformatics analysis, a significantly elevation of NRAV was found in both human GC tissues and cell lines, which indicated the poor prognosis of GC patients. Then, we conducted a series of functional experiments to illustrate the role of NRAV in GC. The results showed that the down-regulation of NRAV exhibited a significant inhibitory effect on GC cell proliferation and migration, while NRAV overexpression promoted GC cell proliferation and migration. Through xenograft mouse tumor model, the suppression of NRAV led to a reduction in the growth of tumor mice, whereas overexpression of NRAV notably enhanced tumor growth. Finally, EFHC1 was revealed as the potential target gene of NRAV. Overall, our findings indicated the promising application of NRAV as a therapeutic target and prognostic biomarker for GC.

Keywords: Gastric cancer; Migration; NRAV; Proliferation; lncRNA.