Identification of mRNA biomarkers in extremely early hypertensive intracerebral hemorrhage (HICH)

Haidong Gao; Jian Zhang; Xinjun Wang; Jixin Shou; Jianye Wang; Peng Yang

doi:10.1186/s12953-024-00237-w

Identification of mRNA biomarkers in extremely early hypertensive intracerebral hemorrhage (HICH)

Proteome Sci. 2024 Nov 30;22(1):12. doi: 10.1186/s12953-024-00237-w.

Authors

Haidong Gao^#¹, Jian Zhang^#¹, Xinjun Wang², Jixin Shou³, Jianye Wang¹, Peng Yang¹

Affiliations

¹ Department of Neurosurgery, Zhengzhou University Fifth Affiliated Hospital, Erqi District, No.3, Rehabilitation Front Street, Zhengzhou, Henan, 45000, China.
² Department of Neurosurgery, Zhengzhou University Fifth Affiliated Hospital, Erqi District, No.3, Rehabilitation Front Street, Zhengzhou, Henan, 45000, China. wangxinjun2045001@163.com.
³ Department of Neurosurgery, Zhengzhou University Fifth Affiliated Hospital, Erqi District, No.3, Rehabilitation Front Street, Zhengzhou, Henan, 45000, China. Shoujx2024001@163.com.

^# Contributed equally.

PMID: 39616335
DOI: 10.1186/s12953-024-00237-w

Abstract

Introduction: Hypertensive intracerebral hemorrhage (HICH) stands out as a critical complication of primary hypertension. Consequently, investigating messenger RNA (mRNA) biomarkers becomes imperative, offering potential targets. This study is conducted for elucidating the expression profile of blood mRNA biomarkers in HICH.

Methods: Twenty-five HICH patients were constituted the HICH group.Twenty-two healthy volunteers recruited and comprised the control group. Peripheral blood cells were extracted to identify candidate mRNA. The identified differential expressions of genes between the two groups were validated, and the potential associations between these differentially expressed genes and adverse events were analyzed. GO and KEGG enrichment of DEGs, Weighted Gene Co-expression Network and Protein Interaction Network were established. target mRNA was screened.

Results: The study identified 3163 differentially expressed genes in HICH. 8 candidate mRNA (SPI1, HK3, HCK, SYK, CD14, FCER1G, CYBB, FGR) were pinpointed. Associations with pathways affecting HICH development included HIF-1 signaling, NF-kappa B signaling, and C-type lectin receptor signaling. In the HICH group, higher expressions of HK3, HCK, SYK, CD14, FCER1G, CYBB, and FGR, and lower SPI1 expression compared to the control group. HICH patients experienced high rates of complications: pulmonary infection (84%), epilepsy (16%), enlarged hematoma (20%), gastrointestinal bleeding (48%), malnutrition (84%), and lower limb deep vein thrombosis (DVT) (12%). Factors contributing to pulmonary infection included age and elevated expression of HCK, SYK, CD14, and FGR. SPI1 was associated with epilepsy, while its lower expression correlated with hematoma enlargement. Gastrointestinal bleeding was linked to increased cerebral hemorrhage. Malnutrition was associated with higher age, and expressions of HK3, HCK, SYK, CD14, FCER1G, CYBB, and FGR. Patients with lower limb DVT had elevated expressions of the identified genes.

Conclusion: In hypertensive intracerebral hemorrhage, there are elevated expressions of HK3, HCK, SYK, CD14, FCER1G, CYBB, and FGR, along with reduced expression of SPI1. Furthermore, age, along with elevated expressions of HCK, SYK, CD14, and FGR, serves as influencing factors contributing to pulmonary infection in patients.

Keywords: High throughput sequencing of next-generation RNA; Hypertensive cerebral hemorrhage; Messenger ribonucleic acid; Weighted gene co-expression network analysis.