Rapid detection of nitrite and Listeria monocytogenes in food based on multiple simulated enzyme activities of Fe3O4-ZnO-MnO2 nanocomposites

Shuyang Sun; Wenteng Qiao; Feng Yin; Wei Mi; LuliangWang; Yuhan Jia; Mengjiao Wang; Xinhui Liu; Dacheng Wang; Daotan Liu; Chao Zhao; Xiuling Song; Yushen Liu; Yue Zhai

doi:10.1016/j.fochx.2024.101968

Rapid detection of nitrite and Listeria monocytogenes in food based on multiple simulated enzyme activities of Fe₃O₄-ZnO-MnO₂ nanocomposites

Food Chem X. 2024 Nov 8:24:101968. doi: 10.1016/j.fochx.2024.101968. eCollection 2024 Dec 30.

Authors

Shuyang Sun¹, Wenteng Qiao¹, Feng Yin², Wei Mi³, LuliangWang¹, Yuhan Jia¹, Mengjiao Wang¹, Xinhui Liu¹, Dacheng Wang¹, Daotan Liu¹, Chao Zhao⁴, Xiuling Song⁴, Yushen Liu^{1

5}, Yue Zhai⁶

Affiliations

¹ School of Food Engineering, Ludong University, Yantai 264025, Shandong, China.
² Yantai Laishan District Center for Disease Control and Prevention, Yantai 264003, Shandong, China.
³ School of Public Health, Binzhou Medical College, Yantai 264003, Shandong, China.
⁴ School of Public Health, Jilin University, Changchun 130021, Jilin, China.
⁵ Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, China.
⁶ School of Nursing Jinlin University, Jinlin University, Changchun 130000, Jinlin, China.

Abstract

Food safety has emerged as a paramount concern in global health, prompting innovative approaches to ensure the safety of people's sustenance. In this study, a novel strategy was devised to fabricate Fe₃O₄-ZnO-MnO₂ hybrid nanobiocatalysts, which exhibited remarkable enzymatic activity surpassing that of Horseradish peroxidase (HRP) catalysis. It demonstrated exceptional proficiency in decomposing 3,3',5,5'-tetramethylbenzidine (TMB) without the need for harsh reaction conditions or the aid of H₂O₂. We established colorimetric detection systems based on Fe₃O₄-ZnO-MnO₂-TMB both for nitrite (NO₂ ^-) and Listeria monocytogenes (LM) in food. Impressively, the detection limit of nitrite reached an astonishingly low level of 0.022 mg L^-1, and the detection limit for LM was determined to be 3.5 cfu mL^-1. These compelling results unequivocally validate the potential of these hybrid nanobiocatalysts to fortify food safety measures. Moreover, they serve as a valuable reference for the colorimetric detection of diverse analytes and the simultaneous detection of multiple targets.

Keywords: Fe3O4-ZnO-MnO2 nanocomposites; Food safety; Listeria monocytogenes; Nitrite; Rapid detection.