Chromatographic and mass spectroscopic guided discovery of Trichoderma peptaibiotics and their bioactivity

Adigo Setargie; Chen Wang; Liwen Zhang; Yuquan Xu

doi:10.1016/j.engmic.2023.100135

Chromatographic and mass spectroscopic guided discovery of Trichoderma peptaibiotics and their bioactivity

Eng Microbiol. 2023 Dec 16;4(2):100135. doi: 10.1016/j.engmic.2023.100135. eCollection 2024 Jun.

Authors

Adigo Setargie^{1

2}, Chen Wang¹, Liwen Zhang¹, Yuquan Xu¹

Affiliations

¹ Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
² Institute of Biotechnology, Bahir Dar University, P.O. Box. 79, Bahir Dar, Ethiopia.

Abstract

Peptaibiotics are linear or cyclic peptide antibiotics characterized by the non-proteinogenic amino acid, alpha-aminoisobutyric acid. They exhibit a wide range of bioactivity against various pathogens. This report presents a comprehensive review of analytical methods for Trichoderma cultivation, production, isolation, screening, purification, and characterization of peptaibiotics, along with their bioactivity. Numerous techniques are currently available for each step, and we focus on describing the most commonly used and recently developed chromatographic and spectroscopic techniques. Investigating peptaibiotics requires efficient culture media, growth conditions, and isolation and purification techniques. The combination of chromatographic and spectroscopic tools offers a better opportunity for characterizing and identifying peptaibiotics. The evaluation of the chemical and biological properties of this compound has also been explored concerning its potential application in pharmaceutical and other industries. This review aims to summarize available data on the techniques and tools used to screen and purify peptaibiotics from Trichoderma fungi and bioactivity against various pathogens.

Keywords: Chromatography; Trichoderma; peptaibiotics; spectroscopy.

Publication types

Review