Abstract
Hybrids between mouse cells and simian virus 40 (SV40)-transformed rat cells were made, and their properties and chromosome constitution were investigated over many generations. Their hybrid nature was confirmed by enzyme studies. During a period of 1 year a loss of 10 to 20% of the total number of chromosomes was observed. The SV40 tumor antigen was present and remained present in the hybrids. The parental and hybrid cells were studied for agglutination with concanavalin A, for growth in soft agar, and for serum requirement. These growth and surface characteristics of the transformed cells appeared in the hybrids.
MeSH terms
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Agglutination
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Animals
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Antigens, Viral / analysis
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Cell Line* / enzymology
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Cell Line* / immunology
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Cell Transformation, Neoplastic*
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Chromosomes
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Clone Cells
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Concanavalin A
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Culture Media
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Electrophoresis
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Fluorescent Antibody Technique
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Hybrid Cells* / cytology
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Hybrid Cells* / enzymology
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Hybrid Cells* / immunology
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Immune Sera
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Indophenol
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L-Lactate Dehydrogenase / analysis
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Mice
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Oxidoreductases / metabolism
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Phosphogluconate Dehydrogenase / analysis
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Rats
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Rats, Inbred Strains
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Simian virus 40* / immunology
Substances
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Antigens, Viral
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Culture Media
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Immune Sera
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Concanavalin A
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Indophenol
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Oxidoreductases
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L-Lactate Dehydrogenase
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Phosphogluconate Dehydrogenase