Effect of simian virus 40 infection on albumin production by hepatocytes cultured in chemically defined medium and plated on collagen and non-collagen attachment surfaces

J Biol Chem. 1984 Aug 10;259(15):9595-602.

Abstract

Simian virus 40 (SV40)-infected rat hepatocytes were tested for albumin production (a marker of differentiated hepatocyte function) by growth in serum-free medium for 30-40 days. We observed a biphasic peak of albumin production, with the first peak at 4-6 and the second at 10-20 days after plating. A 2- to 3-fold increase in the second peak was observed when albumin production by SV40-infected cultures was compared with that by uninfected cultures. No increase in cell number in SV40-infected cultures was observed during this 20-day period. By 25-35 days, albumin production by uninfected cultures ceased but was detected in 50-60% of SV40-infected cultures. SV40-induced stimulation and persistence occurred regardless of the attachment surface and did not require collagen. Transformation occurred regardless of whether stimulation or persistence of albumin production was seen; however, stimulation and persistence were necessary to yield transformed cultures that produced albumin.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Albumins / biosynthesis*
  • Animals
  • Cell Adhesion
  • Cell Transformation, Viral
  • Collagen
  • Culture Media
  • Liver / metabolism*
  • Male
  • Rats
  • Rats, Inbred F344
  • Simian virus 40
  • Time Factors
  • Tumor Virus Infections / metabolism*

Substances

  • Albumins
  • Culture Media
  • Collagen