A new method for detection of small modifications in genomic DNA, applied to the human delta-beta globin gene cluster

Eur J Biochem. 1984 Aug 1;142(3):473-80. doi: 10.1111/j.1432-1033.1984.tb08310.x.

Abstract

Cloned DNA fragments were subcloned in filamentous coliphages fd 103 or M 13; the recombinant single-stranded DNAs were then used to form hybrids with genomic DNA as well as with complementary recombinant single-stranded DNA. Hybrids were submitted to S1-nuclease treatment alone or in combination with restriction enzyme digestions. This method was used to analyze the delta-beta globin gene cluster from the total genomic DNA of a beta 0-thalassemic patient. A modification located approximately 530 base pairs upstream from the cap site of the beta-globin gene was detected in only one thalassemic chromosome of this patient. Sequence analysis have shown that the patient was homozygous for a single nucleoside change (dC----dT) which remains undetected by our hybridization method, leading to a codon 39 nonsense mutation; they have demonstrated too that he was heterozygous for the modification mentioned and detected by S1-nuclease, which corresponds to an additional sequence d(T-A-T-A) in a 52 alternating purine-pyrimidine run, leading to a complex change from d[(A-T)7(T)7] to d[(A-T)11(T)3].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA, Recombinant
  • Endonucleases
  • Genes
  • Globins / genetics*
  • Humans
  • Mutation
  • Nucleic Acid Hybridization
  • Single-Strand Specific DNA and RNA Endonucleases
  • Thalassemia / genetics*

Substances

  • DNA, Recombinant
  • Globins
  • Endonucleases
  • Single-Strand Specific DNA and RNA Endonucleases