Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex

Nature. 1984;312(5993):413-8. doi: 10.1038/312413a0.

Abstract

The complete amino acid sequence of one of the polypeptide chains of the human T3/T-cell receptor complex, the T3 glycoprotein (T3-delta-chain) of relative molecular mass 20,000, was deduced from cDNA clones derived from HPB-ALL cells and a human T-cell clone. Inspection of the 171-amino acid sequence reveals a signal peptide, a 79-amino acid extracellular domain, one transmembrane region and a 44-amino acid intracellular domain which show no sequence homology with members of the T-cell receptor/immunoglobulin/MHC multi-gene family. The T3 delta-chain is coded for by a single-copy gene the expression of which is restricted to T lymphocytes in humans and mice.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cell Line
  • Cloning, Molecular*
  • DNA / isolation & purification*
  • DNA Restriction Enzymes
  • Humans
  • Leukemia, Lymphoid
  • Macromolecular Substances
  • Molecular Weight
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics
  • Receptors, Antigen, T-Cell / genetics*
  • T-Lymphocytes / immunology

Substances

  • Macromolecular Substances
  • RNA, Messenger
  • Receptors, Antigen, T-Cell
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X01451