Isolation and characterization of the trisialogangliosides from bovine adrenal medulla

J Lipid Res. 1983 Jun;24(6):737-45.

Abstract

Trisialogangliosides were isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. Their structures were elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structures of trisialogangliosides, A to D, were identified as follows. A: GT1b, IV3NeuAc, II3 (NeuAc)2-GgOse4Cer. B: GT1b(NeuAc/NeuAc-NeuGc-); IV3NeuAc, II3 (NeuAc alpha 2-8 NeuGc-)GgOse4Cer. C: GT1b (NeuGc/NeuAc-NeuAc-); IV3NeuGc, II3 (NeuAc alpha 2-8 NeuAc-)GgOse4Cer. D: GT1b (NeuAc/NeuGc-NeuGc-); IV3NeuAc, II3 (NeuGc alpha 2-8 NeuGc-)GgOse4Cer. Gangliosides B, C, and D, which contain N-glycolylneuraminic acid, have not previously been reported in the literature.

MeSH terms

  • Adrenal Medulla / analysis*
  • Animals
  • Carbohydrates / analysis
  • Cattle
  • Chromatography, Gas
  • Chromatography, Thin Layer
  • Clostridium perfringens / enzymology
  • Gangliosides / isolation & purification*
  • Mass Spectrometry
  • Methylation
  • Neuraminidase

Substances

  • Carbohydrates
  • Gangliosides
  • trisialoganglioside
  • Neuraminidase