Determination of omeprazole and metabolites in plasma and urine by liquid chromatography

J Chromatogr. 1984 Aug 10;309(2):347-56. doi: 10.1016/0378-4347(84)80042-0.

Abstract

Omeprazole, a substituted benzimidazole and a new gastric acid inhibitor, has been determined in plasma and urine, together with three of its metabolites--the sulphide, the sulphone and the hydroxy compound. The methods comprise extraction from the biological materials with methylene chloride, followed either by direct injection of the extract onto a normal-phase liquid chromatography column or evaporation, dissolution and injection onto a reversed-phase system. The compounds were detected using ultraviolet spectrometry. The absolute recoveries obtained were mostly above 95%. The minimum determinable concentration for omeprazole was 20 nmol/l in plasma (relative standard deviation 10-15%) and 50 nmol/l in urine. The metabolites could also be determined at the same levels.

MeSH terms

  • Anti-Ulcer Agents / analysis*
  • Anti-Ulcer Agents / blood
  • Anti-Ulcer Agents / urine
  • Benzimidazoles / analysis*
  • Benzimidazoles / blood
  • Benzimidazoles / urine
  • Chromatography, Liquid
  • Humans
  • Hydroxylation
  • Omeprazole
  • Spectrophotometry, Ultraviolet
  • Sulfones / analysis

Substances

  • Anti-Ulcer Agents
  • Benzimidazoles
  • Sulfones
  • Omeprazole