Image spread in dry-mounted tritium autoradiographs

J Microsc. 1983 Nov;132(Pt 2):235-8. doi: 10.1111/j.1365-2818.1983.tb04278.x.

Abstract

A comparison has been made of autoradiographs prepared by conventional dipping methods and those prepared from the same material using a dry-mounting method developed for the study of receptor labelling. Resolution was found to be inferior, and image spread greater, in the dry-mounted preparations. The extent to which resolution and image spread were affected depended upon the distance between specimen and emulsion.

MeSH terms

  • Animals
  • Autoradiography / methods*
  • Intestines / ultrastructure
  • Mice
  • Microscopy, Electron / methods*
  • Tongue / ultrastructure
  • Tritium

Substances

  • Tritium