Isolation and identification of Lom-SG-SASP, a salivation stimulating peptide from the salivary glands of Locusta migratoria

D Veelaert; L Schoofs; P Proost; J Van Damme; B Devreese; J Van Beeumen; A De Loof

doi:10.1016/0167-0115(95)00035-A

Isolation and identification of Lom-SG-SASP, a salivation stimulating peptide from the salivary glands of Locusta migratoria

Regul Pept. 1995 Jun 27;57(3):221-6. doi: 10.1016/0167-0115(95)00035-A.

Authors

D Veelaert¹, L Schoofs, P Proost, J Van Damme, B Devreese, J Van Beeumen, A De Loof

Affiliation

¹ Zoological Institute, Katholieke Universiteit Leuven, Belgium.

PMID: 7480871
DOI: 10.1016/0167-0115(95)00035-A

Abstract

From a methanolic extract of about 2500 salivary glands of Locusta migratoria a peptide was isolated which stimulates cAMP production in the salivary glands and salivation. Maldi-TOFMS revealed a mass of 1779 Da. The primary structure of the peptide is NH2-EVGDLFKEWLQGNMN-COOH. The peptide is named Locusta migratoria-Salivary Gland-Salivation Stimulating Peptide (Lom-SG-SASP) because of its simulating effect on salivation. Lom-SG-SASP displays no relevant sequence similarities with any other known peptide from vertebrate or invertebrate sources. The effect of synthetic Lom-SG-SASP on cAMP production in the salivary glands and on salivation is discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Cyclic AMP / biosynthesis*
Grasshoppers / chemistry*
Molecular Sequence Data
Molecular Weight
Salivary Proteins and Peptides / isolation & purification*
Salivary Proteins and Peptides / physiology
Salivation / physiology

Substances

Lom-SG-SASP peptide
Salivary Proteins and Peptides
Cyclic AMP