Three kinds of simplified agar media were reported in this paper, which are easy to prepare and are simple in constituents. The velocity and the quantity of growth of seven strains tested on simplified agar media 309A and 309C were similar to or prevail over those on Lowenstein-Jensen medium and No. 92 potato-soup agar medium. Simplified agar media 309C and 309A could be applied to isolate mycobacteria from sputum and to test the drug susceptibility of M. tuberculosis.