Inverse deuterium kinetic isotope effect for peroxidation in human low-density lipoprotein (LDL): a simple test for tocopherol-mediated peroxidation of LDL lipids

FEBS Lett. 1995 Nov 13;375(1-2):45-9. doi: 10.1016/0014-5793(95)01172-b.

Abstract

alpha-Tocopherol (alpha-TOH) can act as a pro- or antioxidant for isolated ubiquinol-10-free human low density lipoprotein (LDL). We demonstrate that alpha-TOH is a more potent pro-oxidant than other forms of vitamin E for LDL peroxidation initiated by mild fluxes of aqueous peroxyl radicals and low concentrations of Cu2+. A simple deuterium exchange test shows that alpha-TOH switches from pro- to anti-oxidant at Cu2+:LDL ratios > 2.5. The results suggest that this test may be useful to distinguish 'inhibited' peroxidation of emulsion lipids propagated via the lipid peroxyl radical from that mediated via the antioxidant radical.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Deuterium
  • Free Radicals
  • Humans
  • Kinetics
  • Lipid Peroxidation / drug effects*
  • Lipoproteins, LDL / blood*
  • Lipoproteins, LDL / drug effects
  • Male
  • Oxidants / pharmacology*
  • Oxidation-Reduction
  • Oxygen Consumption
  • Peroxides
  • Radioisotope Dilution Technique
  • Reactive Oxygen Species / pharmacology
  • Structure-Activity Relationship
  • Time Factors
  • Vitamin E / pharmacology*

Substances

  • Free Radicals
  • Lipoproteins, LDL
  • Oxidants
  • Peroxides
  • Reactive Oxygen Species
  • Vitamin E
  • perhydroxyl radical
  • Deuterium