Detection of P glycoprotein activity on normal and leukemic CD34+ cells

Leuk Res. 1993 Dec;17(12):1031-5. doi: 10.1016/0145-2126(93)90159-i.

Abstract

Rhodamine 123 is transported by the transmembrane efflux pump P glycoprotein (Pgp). We used this fluorescent dye to study multidrug resistance (MDR) activity in normal and leukemic CD34+ cells. These immature cells had a high degree of MDR activity. Among leukemic cells, CD34+ leukemias had significantly higher MDR activity as compared to CD34- leukemias. Heterogeneous results in cell subpopulations, however, indicate that prognosis should be interpreted in the light of MDR analysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Acute Disease
  • Antigens, CD / analysis*
  • Antigens, CD34
  • Bone Marrow / pathology*
  • Bone Marrow Cells
  • Carrier Proteins / analysis*
  • Flow Cytometry
  • Fluorescent Dyes
  • Follow-Up Studies
  • Humans
  • Immunophenotyping
  • Leukemia / pathology*
  • Membrane Glycoproteins / analysis*
  • Monocytes / cytology
  • Monocytes / pathology*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / classification
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
  • Reference Values
  • Rhodamine 123
  • Rhodamines
  • Time Factors

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antigens, CD
  • Antigens, CD34
  • Carrier Proteins
  • Fluorescent Dyes
  • Membrane Glycoproteins
  • Rhodamines
  • Rhodamine 123