Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen

K Hashinaka; S Hashida; K Hirota; A Saitoh; A Nakata; H Shinagawa; S Oka; K Shimada; E Ishikawa

doi:10.1128/jcm.32.3.819-822.1994

Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen

J Clin Microbiol. 1994 Mar;32(3):819-22. doi: 10.1128/jcm.32.3.819-822.1994.

Authors

K Hashinaka¹, S Hashida, K Hirota, A Saitoh, A Nakata, H Shinagawa, S Oka, K Shimada, E Ishikawa

Affiliation

¹ Department of Biochemistry, Medical College of Miyazaki, Japan.

Abstract

Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was detected by an immunoassay with reverse transcriptase as the antigen and beta-D-galactosidase as the label; this immunoassay was 30-fold more sensitive than the previous immunoassay with peroxidase as the label. The sensitivity and specificity were both 100%. The lowest signal for asymptomatic carriers was 20-fold higher than the highest signal for seronegative subjects.

MeSH terms

Adolescent
Adult
Aged
Antigens
Evaluation Studies as Topic
HIV Antibodies / urine*
HIV Seropositivity / diagnosis
HIV Seropositivity / immunology
HIV-1 / immunology*
Humans
Immunoenzyme Techniques* / statistics & numerical data
Immunoglobulin G / urine
Middle Aged
RNA-Directed DNA Polymerase / immunology
Recombinant Proteins / immunology
Sensitivity and Specificity
beta-Galactosidase

Substances

Antigens
HIV Antibodies
Immunoglobulin G
Recombinant Proteins
RNA-Directed DNA Polymerase
beta-Galactosidase