Primary structure of a novel neuropeptide isolated from the corpora cardiaca of periodical cicadas having adipokinetic and hypertrehalosemic activities

Insect Biochem Mol Biol. 1995 Sep;25(8):929-32. doi: 10.1016/0965-1748(95)00032-q.

Abstract

A new neuropeptide hormone was isolated from the corpora cardiaca of the periodical cicadas, Magicicada species. Primary structure of the peptide as determined by a combination of automated Edman degradation after enzymatic deblocking with pyroglutamate aminopeptidase and mass spectrometry is: pGlu-Val-Asn-Phe-Ser-Pro-Ser-Trp-Gly-Asn-NH2. Synthetic peptide assayed in the green stink bug Nezara viridula caused a 112% increase in hemolymph lipids at a dose of 0.625 pmol, and a 67% increase in hemolymph carbohydrates at a dose of 2.5 pmol. Based on these results we designate this peptide, a first from order Homoptera, as Magicicada species-adipokinetic hormone (Mcsp-AKH).

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Carbohydrate Metabolism
  • Female
  • Hemiptera
  • Insect Hormones / chemistry*
  • Insect Hormones / isolation & purification
  • Insect Hormones / physiology*
  • Insecta / chemistry*
  • Lipid Metabolism
  • Male
  • Molecular Sequence Data
  • Neuropeptides / chemistry*
  • Neuropeptides / isolation & purification
  • Neuropeptides / physiology*
  • Neurosecretory Systems / chemistry*
  • Oligopeptides / physiology*
  • Pyrrolidonecarboxylic Acid / analogs & derivatives

Substances

  • Insect Hormones
  • Neuropeptides
  • Oligopeptides
  • adipokinetic hormone
  • adipokinetic hormone, Magicicada
  • hypertrehalosemic hormone
  • Pyrrolidonecarboxylic Acid