Application of the NASBA nucleic acid amplification method for the detection of human papillomavirus type 16 E6-E7 transcripts

J Virol Methods. 1995 Jul;54(1):75-81. doi: 10.1016/0166-0934(95)00032-p.

Abstract

Using a human papillomavirus type 16 (HPV-16) E6-E7 specific primer set in a nucleic acid sequence-based amplification (NASBA) reaction, detection of HPV-16 transcripts was accomplished in a single enzymatic reaction at 41 degrees C. The NASBA reaction product was visualized either by Northern bolt analysis with an HPV-16 E6-E7-specific 32P-labelled oligonucleotide probe or by a non-radioactive enzyme-linked gel assay (ELGA). In combination with a rapid nucleic acid extraction procedure this method appears to be very suitable for the sensitive and specific detection of HPV-16 transcripts on small amounts of HPV-16-expressing cells of various sources, including cervical smears.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cervix Uteri / pathology
  • Cervix Uteri / virology
  • DNA, Viral / analysis
  • Female
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Amplification Techniques*
  • Oncogene Proteins, Viral / isolation & purification*
  • Papillomaviridae / genetics*
  • Papillomavirus E7 Proteins
  • RNA, Viral / analysis*
  • Repressor Proteins*
  • Tumor Cells, Cultured

Substances

  • DNA, Viral
  • E6 protein, Human papillomavirus type 16
  • Oncogene Proteins, Viral
  • Papillomavirus E7 Proteins
  • RNA, Viral
  • Repressor Proteins
  • oncogene protein E7, Human papillomavirus type 16