A murine model for the immunotherapy of head and neck squamous cell carcinoma

Laryngoscope. 1995 Oct;105(10):1077-80. doi: 10.1288/00005537-199510000-00013.

Abstract

A murine model for the immunotherapy of head and neck cancer was established. The AT-84 tumor, a spontaneously arising oral squamous cell tumor of C3H mice, was evaluated for susceptibility to lymphokine-activated killer (LAK) cells. In vitro chromium-release assays demonstrated that AT-84 is sensitive to LAK-cell-mediated killing. Furthermore, in vivo experiments employing a lung metastasis model demonstrated a 50% reduction in the number of metastases in LAK-cell-treated mice as compared with untreated controls (P2 = .001). These experiments showed that AT-84 is an appropriate model for the immunotherapy of head and neck cancer. This model should be invaluable for further study of the mechanisms involved in immune-mediated therapy of head and neck cancer.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Squamous Cell / immunology
  • Carcinoma, Squamous Cell / therapy*
  • Cytotoxicity Tests, Immunologic / methods
  • Disease Models, Animal*
  • Female
  • Head and Neck Neoplasms / immunology
  • Head and Neck Neoplasms / therapy*
  • Immunotherapy, Adoptive / methods*
  • Interleukin-2 / administration & dosage
  • Killer Cells, Lymphokine-Activated / immunology
  • Lung Neoplasms / immunology
  • Lung Neoplasms / secondary
  • Lung Neoplasms / therapy
  • Mice
  • Mice, Inbred C3H
  • Neoplasm Transplantation
  • Random Allocation
  • Statistics, Nonparametric
  • Tumor Cells, Cultured

Substances

  • Interleukin-2