The purpose of this study was to determine the characteristics of the specific phonomyogram (PMG) of active motor units activated during voluntary isometric contractions. The electromyogram (EMG) and PMG were recorded from 87 anconeus motor units in 14 subjects. The elementary PMG from single motor units was analysed with a spike-triggered averaging technique. The electro-acoustical delay was 3.5 (SD 1.1) ms, which is within the range of values reported in the literature for PMG evoked by motor nerve stimulation. All motor units demonstrated a pattern of impulsive sounds with a duration of 87.2 (SD 10.7) ms. These results would imply that PMG is linked to the contractile activity of the motor units. These results also would suggest that PMG recorded from a contracting muscle in situ reflects the summation of elementary PMG during voluntary contraction more than the overall mechanical properties of the muscle.