PCR detection of the lactocin S structural gene in bacteriocin-producing lactobacilli from meat

J M Rodríguez; L M Cintas; P Casaus; A Suárez; P E Hernández

doi:10.1128/aem.61.7.2802-2805.1995

PCR detection of the lactocin S structural gene in bacteriocin-producing lactobacilli from meat

Appl Environ Microbiol. 1995 Jul;61(7):2802-5. doi: 10.1128/aem.61.7.2802-2805.1995.

Authors

J M Rodríguez¹, L M Cintas, P Casaus, A Suárez, P E Hernández

Affiliation

¹ Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.

Abstract

The lactocin S structural gene (lasA) in seven bacteriocinogenic lactobacilli isolated from fermented sausages was studied. Two degenerate primers were synthesized to amplify a 75-bp fragment of the gene. Three strains amplified the fragment from their plasmid DNA, and hybridization analysis confirmed these results.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacteriocins / genetics*
Base Sequence
Food Microbiology*
Genes, Bacterial*
Lactobacillus / genetics*
Meat
Molecular Sequence Data
Polymerase Chain Reaction*

Substances

Bacteriocins